Part:BBa_J72028:Design

{Bmll76} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

Overlap Extension PCR (wobble construction)

Wobble construction of Bmll76 {<6XHis!}

Wobble mll76/mll77                (52, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll76         {<6XHis!}
----
mll76    Forward wobble for {<6XHis!} (Bmll76)
CCATAGAATTCATGAGATCTCACCAtCAtCACCAtC
mll77    Reverse wobble for {<6XHis!} (Bmll76)
CGTTAGGATCCttaGTGaTGGTGaTGaTGGTGAGATCT